Supplemental Zinc exerts a positive effect against the heat stress damage in intestinal epithelial cells: Assays in a Caco-2 model

Heat stress (HS) lead to intestinal epithelial damage through induction of cellular oxidative stress. Since Zn is an essential nutrient with antioxidant properties that involved in maintaining the barrier function, this study aimed examine effects supplementation epithelium under HS. Caco-2 cells were pre-incubated (50 and 100 µM) prior control (37 °C) or HS conditions (41–42 for 24 h. pretreatment reduced lactate dehydrogenase release, a parameter reflecting cell membrane damage. alleviated ROS generation exposed promoted activity enzymes. Moreover, (specially 50 partly attenuated induced on monolayer integrity as measured by transepithelial electrical resistance phenol red permeability. Therefore, had beneficial evoked high temperatures may be useful nutritional strategy against global warming.